英语翻译电渗流是毛细管电色谱的一个重要特征.对于OTCEC 柱,它的大小取决于毛细管内表面净电荷的数量,体现柱表面的性质

英语翻译
电渗流是毛细管电色谱的一个重要特征.对于OTCEC 柱,它的大小取决于
毛细管内表面净电荷的数量,体现柱表面的性质.图2-3-1 描述在10 mM Tris-HCl缓冲液中,未改性的毛细管和十八烷基改性纳米二氧化硅涂层（LPD 法沉积2次）毛细管（C18-2LPD capillary）的电渗流随pH 变化而变化的关系.无论是空柱还是C18-2LPD 毛细管柱,电渗流的大小都取决于毛细管内表面硅羟基的数目及其离解程度.实验发现,在考察的pH 值范围内,两根毛细管的电渗流均随pH增大而增大.这表明两根毛细管内壁均存在一定数目的硅羟基：pH 增高,壁表面的负电荷密度增大,管壁ζ 电势增大,因而造成了电渗流的增大.pH 9 时,未改性的毛细管电渗流达到最大,说明其表面的硅羟基此时已完全电离.另外从图2-3-1 还可以看出,在同一pH 值条件下,空柱比C18-2LPD 柱有更大的电渗流,说明制备得到的C18-2LPD 柱表面的硅羟基数比空柱少.

The electricity transfusion is a capillary vessel electricity chromatograph key character.Regarding the OTCEC column,its size is decided by capillary vessel internal surface only electric charge quantity,manifests the column surface the nature.Figure 2-3-1 the description in 10 mM in the Tris-HCl buffer solution,not the modified capillary vessel and the octadecyl modification nanometer silicon dioxide coating (the LPD law deposits 2 times) the capillary vessel (C18-2LPD capillary) the electricity transfusion the relations which changes along with the pH change.Regardless of being the spatial column or the C18-2LPD capillary vessel column,the electricity transfusion's size is decided by the capillary vessel internal surface silicon hydroxyl number and the dissociation degree.The experiment discovered that in the inspection pH value scope,two root hair narrow tube's electricity transfusion increases along with pH increases.This indicated that two root hair narrow tube endophragm has certain number the silicon hydroxyl:pH advances,the wall surface's negative charge density increases,the pipe wall Zeta electric potential increases,thus has created electricity transfusion enlargement.pH 9:00,the modified capillary vessel electricity transfusion has not achieved in a big way,explains its superficial the silicon hydroxyl this time already the total ionization.Moreover 2-3-1 may also see from Figure,under the identical pH value condition,the spatial column has the bigger electricity transfusion compared to the C18-2LPD column,explained the preparation obtains the C18-2LPD column surface's silicon hydroxyl number are less than the spatial column.