神马作文网non_ adherent sphere assays for csc（cancer stem cell）中文翻译
来源：学生作业帮 编辑：神马作文网作业帮 分类：英语作业 时间：2024/11/18 12:52:10
non_ adherent sphere assays for csc（cancer stem cell）中文翻译
肿瘤干细胞的形成非粘连球体能力分析
Sphere-forming assays.Cultures were trypsinized and strained through a 40-μm sieve.Tumors were minced,digested in 4 mg/mL collagenase 3 (Worthington,Freehold,NJ),2 mg/mL hyaluronidase (Sigma-Aldrich,Oakville,Ontario,Canada),and 2 mg/mL collagenase IV (Sigma) in serum-free DMEM for 25 min at 37°C,strained through a 40-μm sieve,incubated 5 min at room temperature in 0.75% NH4Cl to lyse RBC,and washed with DMEM + 10% FBS.Single cell suspensions were confirmed microscopically,washed with PBS,and suspended at 1,000 cells/mL in serum-free DMEM supplemented as described ( 4).One hundred microliters of the cell suspension was plated in each of the 30 wells of a 96-well microplate (Nunc).Each well was fed 25 μL serum-free supplemented DMEM every other day for 11 days (from culture) or 14 days (from tumor).Spheres (tight,spherical,nonadherent masses >90 μm in diameter) per well were counted,and at least 50 spheres per group were measured with an ocular micrometer.For secondary sphere-forming assays,primary spheres were dissociated mechanically and processed as in the primary assay.
Sphere-forming assays.Cultures were trypsinized and strained through a 40-μm sieve.Tumors were minced,digested in 4 mg/mL collagenase 3 (Worthington,Freehold,NJ),2 mg/mL hyaluronidase (Sigma-Aldrich,Oakville,Ontario,Canada),and 2 mg/mL collagenase IV (Sigma) in serum-free DMEM for 25 min at 37°C,strained through a 40-μm sieve,incubated 5 min at room temperature in 0.75% NH4Cl to lyse RBC,and washed with DMEM + 10% FBS.Single cell suspensions were confirmed microscopically,washed with PBS,and suspended at 1,000 cells/mL in serum-free DMEM supplemented as described ( 4).One hundred microliters of the cell suspension was plated in each of the 30 wells of a 96-well microplate (Nunc).Each well was fed 25 μL serum-free supplemented DMEM every other day for 11 days (from culture) or 14 days (from tumor).Spheres (tight,spherical,nonadherent masses >90 μm in diameter) per well were counted,and at least 50 spheres per group were measured with an ocular micrometer.For secondary sphere-forming assays,primary spheres were dissociated mechanically and processed as in the primary assay.
non_ adherent sphere assays for csc（cancer stem cell）中文翻译
cancer stem cell是什么?癌的来源细胞吗?
stem cell lines 是什么?
Stem cell cassette
STEM CELL是什么?
stem cell line
stem cell food是什么
squamous cell cancer是什么意思
stem cell fluid是什么意思? 谢谢
can a changed cancer cell attack another cancer cell?
求cell stem cell 文献一篇 "Assessing the Safety of Stem Cell Ther
求几篇英文文献（1）Human mesenchymal stem cell transplantation protec