英语翻译TO PREPARE 1X LIQUID MEDILM1.Measure out 5% less distill

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英语翻译
TO PREPARE 1X LIQUID MEDILM
1.Measure out 5% less distilled water then desired total volume of medium.using a mixing
comtainer that is as close to the final volume as possible
2.Add powdered medium to 15 to 30℃(room temperature) water with gentle stirring （do not heat water）
3.Rinse out inside of package to remove all traces powder
Add 2.0g of NaHCO3 per liter medium
5 Dilute to desired volume with water .Stir until dissolved (Do nt over-mix)
6 Adjust pH of medium to 0.2-0.3 below desired final working pH use of 1N HCL is recommended.(Add slowlu with stirring.)After pH has been adjusted.Keep container closed until medium is filtered.
7.Sterilize immediately by membrane filtration (Positive pressure recommended)
*pH units will usually rise 0.1-0.3 upon filtration

配置 1 份溶液
1.量出比制作溶液体积小 5% 的过滤水（蒸馏水）.尽量用和制取溶液体积相近的容器.
2.加入粉状溶质进入15-30摄氏度（室温）的溶液,稍稍搅拌（勿加热）
3.冲洗溶质包装中的所有粉末进入溶液
4.加入 2.0 克/升小苏打*碳酸钠）进入溶液
5.用蒸馏水稀释溶液直至适当体积,搅拌至溶解
6.把溶液 pH 调制比最终成品pH小0.2-0.3,建议使用 1N （ 1 mol/L )HCl （盐酸）慢慢加入并搅拌.当 pH 调整完毕后,关闭容器知道溶液过滤之后
7.用过膜过滤法消毒（使用正压力）
过滤后 pH 通常会上升 0.1-0.3

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