谁能帮我翻译成英文啊,急

谁能帮我翻译成英文啊,急
摘 要：目的：构建CEA单链抗体-蒜氨酸酶融合基因真核表达载体并在毕赤酵母中进行表达.方法：根据蒜氨酸酶基因序列设计上下游引物,分别引入EcoRI和XbaI限制性内切酶位点.以保存的pGEMP-T-Alliinasee质粒为模版进行PCR扩增.从大肠杆菌中提取pPIczαC质粒,然后,PCR产物和pPIczαC质粒分别用EcoRI和XbaI进行双酶切,构建pPIczαC-Alliinase重组质粒.以保存pGEMP-T-CEA为模板,重新设计引物,分别引入XhoI和EcoRI限制性内切酶位点,进行PCR产物,将PCR产物和pPIczαC-Alliinase分别用EcoRI和XhoI进行双酶切,构建pPIczαC-Alliinase -CEA重组质粒.进行双酶切、PCR鉴定和测序分析.将鉴定正确的pPIczαC-Alliinasee-CEA质粒电转化至毕赤酵母进行表达,SDS-PAGE电泳和Western Blotting检测目的蛋白表达.结果：1. 成功构建CEA单链抗体-蒜氨酸酶融合基因真核表达质粒,大小约为5600bp .2.融合蛋白在毕赤酵母中得到初步表达,分子量约为78KD .结论： CEA单链抗体-蒜氨酸酶融合基因可以在毕赤酵母中获得初步表达.
关键字：癌胚抗原；蒜氨酸酶；单链抗体；基因表达
不要是翻译器翻的 谢谢

Abstract: Objective: to construct CEA scFv antibody-conjugated alliinase fusion gene eukaryotic expression vector and expression in Pichia pastoris expression. Methods: according to the design of alliinase gene sequences upstream and downstream primer, respectively, the introduction of EcoRI and XbaI restriction endonuclease sites. For the preservation of the pGEMP-T-Alliinasee plasmid as template for the PCR amplification. From Escherichia coli extracts pPIcz alpha C plasmid, then, PCR product and pPIcz alpha C plasmid respectively by EcoRI and XbaI double enzyme, pPIcz alpha C-Alliinase recombinant plasmid construction. In order to save pGEMP-T-CEA as template, redesigning primer, respectively, introduce XhoI and EcoRI restriction endonuclease sites, PCR products, the PCR product and pPIcz alpha C-Alliinase respectively by EcoRI and XhoI double digestion, constructing pPIcz alpha C-Alliinase CEA recombinant plasmid. Double enzyme, PCR identification and sequencing analysis. The identification of the correct pPIcz alpha C-Alliinasee-CEA plasmid transformation to the Pichia pastoris expression, SDS-PAGE electrophoresis and Western Blotting detection target protein expression. Results: the successful construction of single chain antibody1CEA alliinase fusion gene eukaryotic expression plasmid, size of about5600bp. The 2fusion protein in Pichia pastoris gets preliminary expression, molecular weight of about78KD. Conclusion: CEA single chain antibody of alliinase gene fusion can be obtained in Pichia pastoris expression.
Keywords: carcinoembryonic antigen; garlic; antibody; gene expression