实验中 control;negative control ;positive control;分别指代什么情况
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实验中 control;negative control ;positive control;分别指代什么情况
还常出现“CK”;是个什么情况?
还常出现“CK”;是个什么情况?
我找到两种解释
Definition of negative control :
1.(Gene regulation) Transcription regulation mediated by factors that block,turn down or turn off transcription.
2 (Experimental) An experiment performed in parallel under the same experimental conditions,exept the variable that is under examination in that particular experiment.
Definition of positive control :
Regulation mediated by a protein that is required for the activation of a transcription unit.
第二种
In an experiment the positive control is an experiment whose result is already known to be positive,it is used to check that nothing went wrong during the procedure.For a PCR detection test it would typically be the amplification of a sample known to yield the correct amplicon.
The negative control is an experiment done with a sample/mock sample that should normally yield a negative result.It allows to check for contamination of the reagents or artifacts that would give false positive.Typically in a PCR that would mean running a PCR reaction with water as a template.
Definition of negative control :
1.(Gene regulation) Transcription regulation mediated by factors that block,turn down or turn off transcription.
2 (Experimental) An experiment performed in parallel under the same experimental conditions,exept the variable that is under examination in that particular experiment.
Definition of positive control :
Regulation mediated by a protein that is required for the activation of a transcription unit.
第二种
In an experiment the positive control is an experiment whose result is already known to be positive,it is used to check that nothing went wrong during the procedure.For a PCR detection test it would typically be the amplification of a sample known to yield the correct amplicon.
The negative control is an experiment done with a sample/mock sample that should normally yield a negative result.It allows to check for contamination of the reagents or artifacts that would give false positive.Typically in a PCR that would mean running a PCR reaction with water as a template.
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