神马作文网英语翻译——物理专业3
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英语翻译——物理专业3
请帮忙翻译这段文字：Estimate of the Number of Proteins per QD-MBP-his Conju?gate. Cy3-labeled MBP-his was utilized to measure the MBP concen?tration in the QD conjugate solution by monitoring the absorption of Cy3 dye at 553 nm (peak of the absorption spectrum, see Figure 2). This is better and more accurate than using the protein absorption at 280 nm, where interference from the large nanocrystal absorption could provide erroneous results. Experimentally, QDs (e.g., 555 nm emitting nanocrystals) were mixed with the molar amount of MBP95C-Cy3 necessary to form QD conjugates with the targeted number of proteins and allowed to self-assemble for ~ 15 min; a control solution with pure MBP95C-Cy3 (no QDs) was also prepared. The two solutions contained the same amounts of proteins as checked by the comparable absorbance at 553 nrn. The conjugate and control solutions were loaded onto an Amicon Centricon spin dialysis tube having a cutoff molecular weight, MW, of 100 kDa (larger than the estimated protein MW of ~45 kDa) and centrifuged at 1000 x g 2 times for 20 min each, with a borate buffer wash between the spins. The amount of dye-labeled MBP in the dialysate was extracted for each solution from the absorbance at 553 nrn. More than 95% of the protein in the control sample passed through the membrane, whereas negligible (less than 3%) dye-labeled MBP95C was collected in the dialysate for the sample containing the QD conjugates. The QDs conjugated to 10 or more MBP95C-Cy3 with a MW of ~500 kDa or larger are blocked by the membrane. This result implies that essentially all the proteins added self-assembled onto the nanocrystal surface to form stable Cy3-labeled QD-MBP95C conjugates." Verification of the number of MBP-his per QD conjugate was derived from QD PL enhancement with increasing number of proteins immobilized on each QD surface as reported in refs 21 and 42. Further proof for QD-MBP conjugate formation as well as an estimate of the number of proteins per conjugate is provided by the FRET data, which will be presented
翻译软件要是能翻得通顺,你以为我还贴到这干什么,谢谢大家.
请帮忙翻译这段文字：Estimate of the Number of Proteins per QD-MBP-his Conju?gate. Cy3-labeled MBP-his was utilized to measure the MBP concen?tration in the QD conjugate solution by monitoring the absorption of Cy3 dye at 553 nm (peak of the absorption spectrum, see Figure 2). This is better and more accurate than using the protein absorption at 280 nm, where interference from the large nanocrystal absorption could provide erroneous results. Experimentally, QDs (e.g., 555 nm emitting nanocrystals) were mixed with the molar amount of MBP95C-Cy3 necessary to form QD conjugates with the targeted number of proteins and allowed to self-assemble for ~ 15 min; a control solution with pure MBP95C-Cy3 (no QDs) was also prepared. The two solutions contained the same amounts of proteins as checked by the comparable absorbance at 553 nrn. The conjugate and control solutions were loaded onto an Amicon Centricon spin dialysis tube having a cutoff molecular weight, MW, of 100 kDa (larger than the estimated protein MW of ~45 kDa) and centrifuged at 1000 x g 2 times for 20 min each, with a borate buffer wash between the spins. The amount of dye-labeled MBP in the dialysate was extracted for each solution from the absorbance at 553 nrn. More than 95% of the protein in the control sample passed through the membrane, whereas negligible (less than 3%) dye-labeled MBP95C was collected in the dialysate for the sample containing the QD conjugates. The QDs conjugated to 10 or more MBP95C-Cy3 with a MW of ~500 kDa or larger are blocked by the membrane. This result implies that essentially all the proteins added self-assembled onto the nanocrystal surface to form stable Cy3-labeled QD-MBP95C conjugates." Verification of the number of MBP-his per QD conjugate was derived from QD PL enhancement with increasing number of proteins immobilized on each QD surface as reported in refs 21 and 42. Further proof for QD-MBP conjugate formation as well as an estimate of the number of proteins per conjugate is provided by the FRET data, which will be presented
翻译软件要是能翻得通顺,你以为我还贴到这干什么,谢谢大家.
人数的估计每QD-MBP-his蛋白质Conju ?门.Cy3-labeled MBP-his是用来测量MBP concen在QD共轭?示威通过监测解决方案的吸收在553米(Cy3染料的顶峰吸收光谱、见图2).这是更好、更准确的比用反蛋白质的吸收,在在280海里的干扰可以提供大纳米晶体吸收引起的错误结果.量子点实验(例如:555海里发射纳米)将与摩尔数量的MBP95C-Cy3必要的形成与目标QD列举一些蛋白质,允许自组装为~ 15分钟,控制解决方案以纯净的MBP95C-Cy3量子点(了准备.这两个方案包含相同的大量的蛋白质是经可与之媲美的吸收在nrn 553.扼和控制解决方案被装载到Amicon Centricon旋转透析管有一个截止分子量、微波、100的kDa(比估计的蛋白质兆瓦的~ 45 kDa)和判读号在1000×2次,每20分钟,之间的缓冲洗硼酸跟着旋转.在MBP染料标记的数量提取自每一个解决方案,从吸收在nrn 553.超过95%的蛋白质在控制样品经过了膜,而微不足道的(小于3%)染料标记MBP95C被收录在了样品费自包含QD它.量子点的分子和MBP95C-Cy3 10个或更多,kDa∼500兆瓦的或更大的是被堵了膜.这个结果意味着本质上的所有蛋白质补充说自组装到纳米晶体表面,形成稳定的Cy3-labeled列举QD-MBP95C.”验证的数量每MBP-his QD共轭来自于QD PL力度并和越来越多的蛋白质固定在每QD面参考文献报道21岁,42.为进一步的证明QD-MBP共轭形成作为一个人数的估计每共轭是由蛋白质