英语翻译The plasmid pRL-null,which encodes Renilla luciferase,wa
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英语翻译
The plasmid pRL-null,which encodes Renilla luciferase,was purchased from Promega.Plasmids expressing a dominant-active (da)-Ras (pSV/H-RasV12) and dominant-negative (dn)-Ras (pSV/H- RasN17) were kindly provided by Dr.P.-G.Suh (Department of Life Science,Pohang University of Science and Technology,Korea).The plasmids for dn-Raf1 (pSV/Raf1 DN),dn-MEK1 (pCGN1/MEK DN),dn- Erk2 (pHA-Erk2 K52R),dn-JNK1 (pSRα/HA-JNK T183A/Y185F),and dn-p38 kinase (pCDNA3/flag-p38 T180A/Y182F) were kindly provided by Dr.D.S.Min (Department of Molecular Biology,College of Natural Science,Pusan National University,Korea).dn-Egr-1 (pCDNA3.1/ Egr1N372) was generated as previously described (Gosslar et al.,1999).
The plasmid pRL-null,which encodes Renilla luciferase,was purchased from Promega.Plasmids expressing a dominant-active (da)-Ras (pSV/H-RasV12) and dominant-negative (dn)-Ras (pSV/H- RasN17) were kindly provided by Dr.P.-G.Suh (Department of Life Science,Pohang University of Science and Technology,Korea).The plasmids for dn-Raf1 (pSV/Raf1 DN),dn-MEK1 (pCGN1/MEK DN),dn- Erk2 (pHA-Erk2 K52R),dn-JNK1 (pSRα/HA-JNK T183A/Y185F),and dn-p38 kinase (pCDNA3/flag-p38 T180A/Y182F) were kindly provided by Dr.D.S.Min (Department of Molecular Biology,College of Natural Science,Pusan National University,Korea).dn-Egr-1 (pCDNA3.1/ Egr1N372) was generated as previously described (Gosslar et al.,1999).
该质粒pRL - null,它编码海肾荧光素酶,购自试剂盒.质粒表达主导积极的(大),拉斯(pSV/H-RasV12)和显性负(DN)的-拉斯(pSV/H- RasN17)热心提供了博士P.-G.瑞(生命科学系,浦项科技,高丽大学).为DN的质粒,Raf1(pSV/Raf1的DN)的DN -激酶1(pCGN1/MEK的DN),肾病,Erk2素(PHA - Erk2 K52R),的DN - JNK1的(pSRα/医管局化JNK T183A/Y185F)和DN - p38激酶(pCDNA3/flag-p38 T180A/Y182F)热心提供了局副局长闵博士(分子生物学,自然科学,釜山国立大学部大学,韩国).的DN - Egr - 1的(pCDNA3.1 / Egr1N372)生成如前所述(Gosslar等.,1999).
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