神马作文网英语翻译We have cloned three novel histone genes using antibodie
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英语翻译
We have cloned three novel histone genes using antibodies that recognize onlynuclei of the male gametic (generative and sperm) cells of Lilium longiflorum.The deduced aminoacid sequence of each clone shows only between 40% and 50% identity with the H2A,H2B and H3 somaticcore histones of other plant species.Transcripts of these genes were first detected in bicellularpollen soon after microspore mitosis,and their mRNAs,as revealed by in situ hybridization,wereobserved only in the cytoplasm of the generative cells.As expression of these three genes wasspecific to generative cells within the bicellular pollen,we designated the clones gH2A,gH2B andgH3.Immunocytochemistry further revealed that the proteins encoded by these genes accumulated inthe elongating and condensing generative nucleus during development of bicellular pollen,and weremost abundant in the two sperm nuclei within an elongated pollen tube.We therefore propose thatthese male gamete-specific core histones contribute to chromatin condensation of male gametes or tochromatin remodeling,and result in the repression of gene expression in male gametes.
We have cloned three novel histone genes using antibodies that recognize onlynuclei of the male gametic (generative and sperm) cells of Lilium longiflorum.The deduced aminoacid sequence of each clone shows only between 40% and 50% identity with the H2A,H2B and H3 somaticcore histones of other plant species.Transcripts of these genes were first detected in bicellularpollen soon after microspore mitosis,and their mRNAs,as revealed by in situ hybridization,wereobserved only in the cytoplasm of the generative cells.As expression of these three genes wasspecific to generative cells within the bicellular pollen,we designated the clones gH2A,gH2B andgH3.Immunocytochemistry further revealed that the proteins encoded by these genes accumulated inthe elongating and condensing generative nucleus during development of bicellular pollen,and weremost abundant in the two sperm nuclei within an elongated pollen tube.We therefore propose thatthese male gamete-specific core histones contribute to chromatin condensation of male gametes or tochromatin remodeling,and result in the repression of gene expression in male gametes.
我们已克隆了3个新的组蛋白基因,利用抗体确认onlynuclei的雄性配子（生成和精子）细胞的铁炮百合.推导的氨基酸序列,每个克隆显示,只有40 ％至50 ％的身份与h2a ,h2b和H3的somaticcore组蛋白的其他植物物种.誊这些基因的首次发现,在bicellularpollen后不久,小孢子有丝分裂,他们的基因,为揭示原位杂交,wereobserved只在细胞质中生成细胞.为表达这三个基因wasspecific以生成细胞内bicellular花粉,我们指定了无性系gh2a ,gh2b andgh3 .免疫细胞化学进一步显示,该编码的蛋白是由这些基因的累积inthe拔节和冷凝生成核的开发过程中bicellular花粉,并weremost丰富,在两个精子细胞核内的一个狭长花粉管.因此,我们建议thatthese雄性配子特定核心蛋白有助于染色质浓缩的雄性配子或tochromatin重塑,并导致镇压的基因表达的雄配子
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