英语翻译An aliquot of plasma (0.4 mL) was added to 0.4 mL of wat

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英语翻译
An aliquot of plasma (0.4 mL) was added to 0.4 mL of water,and the resulting solution was applied to a previously activated (3 mL of methanol followed by 6 mL of water) 100-mg Oasys cartridge (Waters,Milford,Mass).
An aliquot of plasma (0.4 mL) was added to 0.4 mL of water,and the resulting solution was applied to a previously activated (3 mL of methanol followed by 6 mL of water) 100-mg Oasys cartridge (Waters,Milford,Mass).After washing with 3 mL of water and 3 mL of water-methanol (v/v,60:40),the flavonoid fraction was eluted with 3 mL of methanol,dried under nitrogen,and dissolved in 0.05 mL of methanol.The resulting solution was centrifuged at 1000g for 1 minute,and 20 μL was injected in HPLC system.

一个aliquot血浆（ 0.4毫升）加0.4毫升水,以及由此产生的解决方案适用于先前激活（ 3毫升的甲醇其次是6毫升水） 100毫克Oasys墨盒（水,米尔福德,大众） .清洗后的3毫升水和3毫升水,甲醇（五/五,60:40 ） ,黄酮分数洗脱3毫升的甲醇,干燥氮气,并溶解于0.05毫升的甲醇.由此产生的解决方案是在1000离心1分钟,20 μL注入高效液相色谱系统

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