根据ProtScale 预测结果,如何判断蛋白质序列的疏水性
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根据ProtScale 预测结果,如何判断蛋白质序列的疏水性
Using the scale Hphob./ Kyte & Doolittle,the individual values for the 20 amino acids are:
Ala:1.800 Arg:-4.500 Asn:-3.500 Asp:-3.500 Cys:2.500 Gln:-3.500
Glu:-3.500 Gly:-0.400 His:-3.200 Ile:4.500 Leu:3.800 Lys:-3.900
Met:1.900 Phe:2.800 Pro:-1.600 Ser:-0.800 Thr:-0.700 Trp:-0.900
Tyr:-1.300 Val:4.200 :-3.500 :-3.500 :-0.490
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Weights for window positions 1,..,21,using linear weight variation model:
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
edge center edge
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MIN:-1.724
MAX:1.052
请问怎么判断此序列是不是具有疏水性?从图中看是数值要达到多少值才行吗?具体是多少?
Using the scale Hphob./ Kyte & Doolittle,the individual values for the 20 amino acids are:
Ala:1.800 Arg:-4.500 Asn:-3.500 Asp:-3.500 Cys:2.500 Gln:-3.500
Glu:-3.500 Gly:-0.400 His:-3.200 Ile:4.500 Leu:3.800 Lys:-3.900
Met:1.900 Phe:2.800 Pro:-1.600 Ser:-0.800 Thr:-0.700 Trp:-0.900
Tyr:-1.300 Val:4.200 :-3.500 :-3.500 :-0.490
________________________________________
Weights for window positions 1,..,21,using linear weight variation model:
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
edge center edge
________________________________________
MIN:-1.724
MAX:1.052
请问怎么判断此序列是不是具有疏水性?从图中看是数值要达到多少值才行吗?具体是多少?
可能是比较中性,Val疏水值都到4.200了,MAX才1.052
mark,望高人解答.
mark,望高人解答.