英语翻译RESULTSIsolation and identification of bacteria.Biodegra

英语翻译
RESULTS
Isolation and identification of bacteria.Biodegradation of
o-DCB was detected in the mixed culture after 14 months of
enrichment in the presence of o-DCB.A pure culture was
obtained by repeated subculture on minimal salts agar plates
supplemented with 0.005% yeast extract,with o-DCB supplied
in the vapor phase.The isolate was a gram-negative rod
which was motile by means of one to two polar flagella.It
was oxidase and catalase positive and nonfermentative,and
required biotin and either methionine or cysteine for optimum
growth.Glucose,lactose,and maltose served as
growth substrates.It reduced nitrate to nitrogen,accumulated
poly-,-hydroxybutyrate,and hydrolyzed Tween 80and starch.No pigments were produced,and the organism
did not hydrolyze gelatin or grow at 37°C.Nitrate was the
preferred source of nitrogen.On the basis of these characteristics,
it was tentatively identified as a Pseudomonas
species and designated JS100.
The isolate grew on chlorobenzene as well as o-DCB.
p-DCB,m-DCB,3,4-dichlorophenol,benzene,and toluene
did not support growth under the conditions tested.
The inclusion of bromthymol blue in the medium of strain
JS100 growing on o-DCB or chlorobenzene permitted the
detection of a color change in the medium,which was
indicative of acid production.Growth of the organism in
liquid culture was also paralleled by a drop in the pH of the
medium.Examination of the culture filtrate from cells grown
in chloride-free medium indicated that chloride was released
from the o-DCB.The minimum doubling time for JS100
grown in batch culture with o-DCB supplied in the vapor
phase was 5.5 h.
请不要用一些所谓的翻译工具来浪费您宝贵的时间，我需要达人。

饥渴欲望,强烈呼求加分HOHO...
（试验）结果
细菌的分离鉴别.
在加入邻二氯苯浓缩后经过14个月浓缩的混合培养物当中能够检测出邻二氯苯的生化降解物,通过重复多次在含0.005%的酵母提取素微盐琼脂板上培养,并以气态方式供以邻二氯苯,就可以获得纯培养物.该分离物是以单一或两极鞭毛方式运动的阴性革兰氏杆菌的聚集,富含氧化酵素以及catalase（这个不清楚是什么）同时未经发酵,加入维生素H、蛋氨酸或半胱氨酸两种其中一类,调节至最佳生长条件,以葡萄糖、乳糖和麦芽糖作为其培养基,它减少了硝酸钾和氮元素的含量,增加了羟基丁酸聚合物及80－淀粉之间的水解物,没有产生色素,同时在37摄氏度下有机物也没有形成凝胶,硝酸钾是首选氮源,基于以上特点,可以初步鉴定其为假单胞菌物种并将其命名为JS100.
该分离物可在含氯苯、邻二氯苯、对二氯苯、间二氯苯、3,4 -二氯苯酚的培养基上生长,苯和甲苯在曾经试验过的条件下不支持该菌种生长.
在邻二氯苯或氯苯环境中生长的JS100的培养基里头加入溴蓝就可以通过检测培养基的颜色变化以指示酸性物质产生量,有机物增加同样可以通过在液态培养物的培养基的PH值微量变化来同步指示,通过无氯培养基中生长的细胞其培养物的过滤物的检测,氯化物经邻二氯苯产生,在混合培养基中辅以气态形式添加邻二氯苯的方式重复培养JS100的最少时间为5.5个小时.